Mefuta e Robeli ea Likokoana-hloko tsa ho Phefumoloha
Lebitso la sehlahisoa
HWTS-RT184-Mefuta e Robeli ea Likokoana-hloko tsa ho Phefumoloha Nucleic Acid Detection Kit (Fluorescence PCR)
Epidemiology
Tšoaetso ea phefumoloho ke mofuta o tloaelehileng ka ho fetisisa oa lefu la batho, le ka hlahang ho bong leha e le bofe, lilemo le sebaka, 'me ke e' ngoe ea lisosa tsa bohlokoa ka ho fetisisa tsa ho kula le lefu lefatšeng.[1]. Likokoana-hloko tse tloaelehileng tsa phefumoloho li kenyelletsa kokoana-hloko ea ntaramane A (IFV A), kokoana-hloko ea ntaramane ea B (IFV B), kokoana-hloko ea syncytial ea ho hema, adenovirus, human metapneumovirus, rhinovirus, kokoana-hloko ea parainfluenza (I/II/III) le mycoplasma pneumoniae, joalo-joalo.[2,3]. Matšoao a kliniki le matšoao a bakoang ke tšoaetso ea pampiri ea ho hema a batla a tšoana, empa tšoaetso e bakoang ke likokoana-hloko tse fapaneng e na le mekhoa e fapaneng ea phekolo, liphello tsa phekolo le nako ea lefu lena.[4,5]. Hona joale, mekhoa e ka sehloohong ea ho lemoha ka laboratori ea likokoana-hloko tsa phefumoloho e kenyelletsa: ho itšehla thajana ha kokoana-hloko, ho lemoha antigen le ho lemoha ha nucleic acid. Setsi sena se lemoha le ho khetholla li-nucleic acid tse khethehileng ho batho ba nang le matšoao le matšoao a tšoaetso ea ho hema, hammoho le liphello tse ling tsa kliniki le tsa laboratori ho thusa ho hlahloba tšoaetso ea kokoana-hloko ea phefumoloho.
Tekheniki Parameters
| Polokelo | 2-8℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | lesela la Oropharyngeal; Nasopharyngeal swab |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | 200 likopi / mL |
| Tse khethehileng | Cross reactivity: Ha ho na cross reactivity between the kit le Boca virus, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, maselese kokoana-hloko, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococ pneumoniae Streptococcusecus Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium lefuba, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Morara, Streptococcus calictococcus, salictococcus Corynebacterium, DNA ea motho. Teko ea ho kena-kenana: Khetha mucin (60mg/mL), mali a motho (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride e nang le 5% preservative (20mg/mL), beclomethasone (20mg/mL), dexamethasone/mL), fluomethasone (20mg/mL) triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), peramivir (1mg/mL) (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Liphetho li bonts'a hore lintho tse kena-kenanang ho concentra e kaholimo. |
| Lisebelisoa tse sebetsang | E sebetsa ho mofuta oa mochini oa tlhahlobo oa I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Real-Time PCR Detection Systems (FQD-96A, Hangzhou Bioer technology), MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou, CRF9, Molar) Sistimi, BioRad CFX Opus 96 Real-Time PCR System. E sebetsa ho mochini oa ho lemoha oa mofuta oa II: EudemonTM AIO800 (HWTS-EQ007) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (e ka sebelisoang ka Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-80) e sebelisa EWTS-30TM AIO800 (HWTS-EQ007)) ka Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bolumo ea sampole e ntšitsoeng ke 200μL mme bophahamo ba molumo o khothaletsoang ke 150μL.
