Mefuta e Robeli ea Livaerase tsa ho Phefumoloha
Lebitso la sehlahisoa
HWTS-RT184-Mefuta e Robedi ea Livaerase tsa Phefumoloho Kit ea ho Fumana Nucleic Acid (Fluorescence PCR)
Thuto ea mafu a seoa
Tšoaetso ea phefumoloho ke mofuta o tloaelehileng haholo oa lefu la batho, le ka hlahang bong bofe kapa bofe, lilemo le sebaka sefe kapa sefe, 'me ke e' ngoe ea lisosa tsa bohlokoa ka ho fetisisa tsa bokuli le lefu lefatšeng.[1]. Likokoana-hloko tse tloaelehileng tsa ho hema li kenyelletsa kokoana-hloko ea ntaramane A (IFV A), kokoana-hloko ea ntaramane B (IFV B), kokoana-hloko ea ho hema ea syncytial, adenovirus, human metapneumovirus, rhinovirus, parainfluenza virus (I/II/III) le mycoplasma pneumoniae, jj.[2,3]Matšoao a kliniki le matšoao a bakoang ke tšoaetso ea phefumoloho a batla a tšoana, empa tšoaetso e bakoang ke likokoana-hloko tse fapaneng e na le mekhoa e fapaneng ea kalafo, litlamorao tsa pholiso le tsela eo lefu le hlahang ka eona.[4,5]Hona jwale, mekgwa e meholo ya ho lemoha dikokwana-hloko tsa ho hema ka laboratoring e kenyeletsa: ho arolwa ha dikokwana-hloko, ho lemoha di-antigen le ho lemoha nucleic acid. Kit ena e lemoha le ho hlwaya di-nucleic acid tse ikgethang tsa kokwana-hloko ho batho ba nang le matshwao le matshwao a tshwaetso ya ho hema, hammoho le diphetho tse ding tsa kliniki le tsa laboratori ho thusa ho hlwaya tshwaetso ya kokwana-hloko ya ho hema.
Li-parameter tsa Tekheniki
| Polokelo | 2-8℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Swab ea molomo; Swab ea molomo |
| Ct | IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5.0% |
| LoD | Likopi tse 200/mL |
| Ho hlaka | Ho arabela ka tsela e fapaneng: Ha ho na ho arabela ka tsela e fapaneng pakeng tsa kit le vaerase ea Boca, Cytomegalovirus, vaerase ea Epstein-Barr, vaerase ea Herpes simplex, vaerase ea varicella zoster, vaerase ea mumps, Enterovirus, vaerase ea measles, coronavirus ea batho, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Lactobacillus, Corynebacterium, DNA ea genomic ea batho. Teko ea ho sitisa: Khetha mucin (60mg/mL), mali a motho (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), sodium chloride e nang le 5% ea sebolaya-mafu (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), fluniacetone (20μg/mL), triamcinolone (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), menthol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mupirocin (20mg/mL), tobramycin (0.6mg/mL), oseltamivir (60ng/mL), ribavirin (10mg/L), Liphetho li bontšitse hore lintho tse sitisang tse ka holimo |
| Lisebelisoa tse Sebetsang | E sebetsa ho reagent ea ho lemoha mofuta oa I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Mekhoa ea PCR ea Nako ea 'Nete, Mekhoa ea PCR ea Nako ea 'Nete ea SLAN-96P (Hongshi Medical Technology Co., Ltd.), LineGene 9600 Plus Mekhoa ea ho Fumana PCR ea Nako ea 'Nete (FQD-96A, theknoloji ea Hangzhou Bioer), MA-6000 Sesebelisoa sa ho Fuputsa Thermal sa Nako ea 'Nete (Suzhou Molarray Co., Ltd.), Sistimi ea PCR ea BioRad CFX96, Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX Opus 96. E sebetsa ho reagent ea ho lemoha mofuta oa II: EudemonTM AIO800 (HWTS-EQ007) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Phallo ea Mosebetsi
Sete ea Macro & Micro-Test Viral DNA/RNA (HWTS-3017) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), le Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8) (e ka sebelisoang le EudemonTM AIO800 (HWTS-EQ007)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Bophahamo ba sampole e ntšitsoeng ke 200μL mme bophahamo bo kgothaletswang ba elution ke 150μL.
