Borrelia Burgdorferi Nucleic Acid
Lebitso la sehlahisoa
HWTS-OT076 Borrelia Burgdorferi Nucleic Acid Detection Kit(Fluorescence PCR)
Setifikeiti
CE
Epidemiology
Lefu la Lyme le bakoa ke tšoaetso ea Borrelia burgdorferi 'me haholo-holo le fetisoa pakeng tsa liphoofolo tse ngata, pakeng tsa liphoofolo tse amohelang batho le liboseleise tse thata. Pathogen Borrelia burgdorferi e ka baka batho ba Erythema chronicum migrans, hammoho le mafu a amanang le litsamaiso tse ngata tse kang pelo, methapo le manonyeletso, joalo-joalo, 'me lipontšo tsa kliniki li fapane. Ho ea ka ntshetsopele ya mafu nete hore, e ka aroloa ka tshwaetso pele localized, tshwaetso mahareng ajoa le morao tshwaetso phehellang, e leng kotsi e tebileng ho bophelo bo botle ba baahi. Ka hona, tlhahlobong ea bongaka ea Borrelia burgdorferi, ho bohlokoa haholo ho theha mokhoa o bonolo, o tobileng le o potlakileng oa tlhahlobo ea etiological ea Borrelia burgdorferi.
Channel
| FAM | DNA ea Borrelia burgdorferi |
| VIC/HEX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | Mohlala oa mali kaofela |
| Tt | ≤38 |
| CV | ≤5.0% |
| LoD | 500 Likopi / mL |
| Lisebelisoa tse sebetsang | ABI 7500 Real-Nako PCR Systems ABI 7500 Fast Real-Nako PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Khetho ea 1.
QIAamp DNA Blood Midi Kit ka Qiagen (51185).It e lokela ho ntšoaka ho latelana ka thataho ea ho taeo, 'me molumo o khothalletsoang oa elution ke100μL
Khetho ea 2.
MaliGDNA e matlaExtraction kit (DP318,Che.: JingchangSesebediswa Record20210062) e hlahisitsoeng ke Tiangen Biochemical Technology (Beijing) Co., Ltd.. It e lokela ho ntšoaka ho latelana ka thataho ea ho taeo, 'me molumo o khothalletsoang oa elution ke100μL
Khetho ea 3.
Wizard® Genomic DNA Purification Kit (A1120) ka Promega.It e lokela ho ntšoaka ho latelana ka thataho ea ho taeo, 'me molumo o khothalletsoang oa elution ke100μL
