ALDH Liphatsa tsa lefutso tsa polymorphism
Lebitso la sehlahisoa
HWTS-GE015Kiti ea ho Fumana Polymorphism ea Liphatsa tsa Lefutso ea ALDH (ARMS -PCR)
Thuto ea mafu a seoa
Lefu la ALDH2 (acetaldehyde dehydrogenase 2), le fumaneha ho chromosome ea motho 12. ALDH2 e na le ts'ebetso ea esterase, dehydrogenase le reductase ka nako e le 'ngoe. Lithuto li bontšitse hore ALDH2 ke enzyme ea metabolism ea nitroglycerin, e fetolang nitroglycerin hore e be nitric oxide, ka hona e phomotsa methapo ea mali le ho ntlafatsa mathata a phallo ea mali. Leha ho le joalo, ho na le li-polymorphisms ho gene ea ALDH2, tse shebaneng haholo le Asia Bochabela. ALDH2*1/*1 GG ea mofuta oa hlaha e na le bokhoni bo matla ba metabolism, ha mofuta oa heterozygous o na le 6% feela ea ts'ebetso ea enzyme ea mofuta oa hlaha, 'me mofuta oa homozygous mutant o na le ts'ebetso ea enzyme e batlang e se na letho, ka metabolism e fokola haholo 'me e ke ke ea fihlela phello e lakatsehang, ka hona e baka kotsi 'meleng oa motho.
Mokero
| FAM | ALDH2 |
| ROX | Taolo ea Ka Hare |
Li-parameter tsa Tekheniki
| Polokelo | ≤-18℃ |
| Nako ea ho phela | Likhoeli tse 12 |
| Mofuta oa Sampole | Mali a sa kang a bokellana maling a EDTA |
| CV | <5.0% |
| LoD | 103Likopi/mL |
| Lisebelisoa tse Sebetsang | Sistimi ea PCR ea Nako ea 'Nete e Sebelisitsoeng ea 7500 Mekhoa ea PCR e Potlakileng ea Li-Biosystem tse 7500 tsa Nako ea 'Nete QuantStudio®Mekhoa e 5 ea PCR ea Nako ea Sebele Mekhoa ea PCR ea Nako ea Sebele ea SLAN-96P Leseli la ho Tsamaisa Li-Cycler®Sistimi ea PCR ea Nako ea 'Nete ea 480 Sistimi ea ho lemoha PCR ea LineGene 9600 Plus ea Nako ea 'Nete MA-6000 Nako ea Sebele ea Bongata ba Thermal Cycler Sistimi ea PCR ea BioRad CFX96 ea Nako ea 'Nete Sistimi ea PCR ea BioRad CFX Opus 96 ea Nako ea 'Nete |
Phallo ea Mosebetsi
Li-reagent tse khothaletsoang tsa ho ntša mali: sebelisa Blood Genome DNA Extraction Kit (DP318) ka Tiangen Biotech (Beijing) Co., Ltd. kapa Blood Genome Extraction Kit (A1120) ka Promega ho ntša EDTA maling a sa kang a etsa mali hantle.
Li-reagent tse khothaletsoang tsa ho ntša: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) ke Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Ho ntša ho lokela ho etsoa ho latela litaelo ka tieo. Bophahamo bo khothaletsoang ba ho tlosa ke100μL.
