ALDH Genetic Polymorphism
Lebitso la sehlahisoa
HWTS-GE015ALDH Genetic Polymorphism Detection Kit (ARMS -PCR)
Epidemiology
ALDH2 gene (acetaldehyde dehydrogenase 2), e fumana chromosome ea motho 12. ALDH2 e na le ts'ebetso ea esterase, dehydrogenase le reductase ka nako e le 'ngoe. Liphuputso li bontšitse hore ALDH2 ke enzyme ea metabolism ea nitroglycerin, e fetolang nitroglycerin ho nitric oxide, kahoo e khatholla methapo ea mali le ho ntlafatsa mathata a phallo ea mali. Leha ho le joalo, ho na le li-polymorphisms mofuteng oa ALDH2, o tsepamisitsoeng haholo Asia Bochabela. Mofuta oa hlaha oa ALDH2 * 1/* 1 GG o na le matla a matla a metabolism, athe mofuta oa heterozygous o na le 6% feela ea ts'ebetso ea enzyme ea hlaha, 'me mofuta oa homozygous mutant o batla o na le ts'ebetso ea enzyme, e nang le metabolism e fokolang haholo mme e sitoa ho fihlela phello e lakatsehang, kahoo e baka kotsi 'meleng oa motho.
Channel
| FAM | ALDH2 |
| ROX | Taolo ea ka hare |
Tekheniki Parameters
| Polokelo | ≤-18℃ |
| Shelf-bophelo | Likhoeli tse 12 |
| Mofuta oa Mohlala | EDTA mali a anticoagulated |
| CV | <5.0％ |
| LoD | 103Likopi/mL |
| Lisebelisoa tse sebetsang | Lisebelisoa tsa Biosystems 7500 tsa Nako ea 'Nete ea PCR Applied Biosystems 7500 Fast Real-Time PCR Systems QuantStudio®Litsamaiso tse 5 tsa nako ea 'nete tsa PCR Litsamaiso tsa PCR tsa nako ea sebele tsa SLAN-96P LightCycler®Sistimi ea 480 ea Nako ea 'Nete ea PCR LineGene 9600 Plus Nako ea 'Nete ea PCR ea ho Fumana Sistimi MA-6000 Real-Nako Quantitative Thermal Cycler Sistimi ea PCR ea Nako ea 'Nete ea BioRad CFX96 BioRad CFX Opus 96 Real-Nako PCR System |
Phallo ea Mosebetsi
Li-reagents tse khothalelitsoeng: sebelisa Blood Genome DNA Extraction Kit (DP318) ke Tiangen Biotech (Beijing) Co.,Ltd. kapa Blood Genome Extraction Kit (A1120) ka Promega ho ntša EDTA anticoagulated mali Genomic DNA.
Li-reagents tse khothalelitsoeng: Macro & Micro-Test General DNA/RNA Kit (HWTS-3019) (e ka sebelisoang le Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-EQ011)) by Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Molumo o khothaletsoang oa elution ke100μL
