Liphetoho tsa lintlha tse hlahang ka har'a jini ea KRAS li amahanngoa le mefuta e fapaneng ea lihlahala tsa batho, ka sekhahla sa liphetoho tse ka bang 17%-25% ho pholletsa le mefuta ea hlahala, 15%-30% kankereng ea matšoafo, le 20%-50% kankereng ea mala a maholo. Liphetoho tsena li khanna khanyetso ea kalafo le tsoelo-pele ea hlahala ka mokhoa oa bohlokoa: protheine ea P21 e kentsoeng ke KRAS e sebetsa ka tlase ho tsela ea lipontšo tsa EGFR. Hang ha KRAS e fetotsoe, e lula e kenya tšebetsong lipontšo tse tlase, e leng se etsang hore mekhoa ea phekolo e shebaneng le EGFR e se ke ea sebetsa hantle 'me e lebisa ho ata ha lisele tse kotsi. Ka lebaka leo, liphetoho tsa KRAS li amahanngoa le khanyetso ho li-inhibitor tsa tyrosine kinase tsa EGFR kankereng ea matšoafo le ho phekolo ea antibody ea anti-EGFR kankereng ea mala a maholo.

Ka 2008, National Comprehensive Cancer Network (NCCN) e thehile litataiso tsa bongaka tse khothalletsang tlhahlobo ea phetoho ea KRAS bakeng sa bakuli bohle ba nang le kankere ea mala a maholo (mCRC) e metastatic pele ho kalafo. Litataiso li totobatsa hore boholo ba liphetoho tsa KRAS tse sebetsang li etsahala ho codons 12 le 13 ea exon 2. Kahoo, ho lemoha phetoho ea KRAS ka potlako le ka nepo ho bohlokoa bakeng sa ho tataisa phekolo e nepahetseng ea bongaka.

Hobaneng Teko ea KRAS e le ea Bohlokoa hoMestastaticColorectalCancer(mCRC)

Kankere ea mala a maholo (CRC) ha se lefu le le leng empa ke pokello ea mefuta e fapaneng ea limolek'hule. Liphetoho tsa KRAS—tse teng ho bakuli ba CRC ba ka bang 40-45%—li sebetsa e le phetoho e sa khaotseng, li khothalletsa kholo ea kankere ntle le matšoao a kantle. Bakeng sa bakuli ba nang le mCRC, boemo ba KRAS bo khetholla katleho ea li-antibodies tsa monoclonal tse khahlanong le EGFR tse kang Cetuximab le Panitumumab:

KRAS ea Mofuta o Hlaha:Ho ka etsahala hore bakuli ba rue molemo kalafong e khahlanong le EGFR.

KRAS e fetotsoeng:Bakuli ha ba fumane molemo ho tsoa ho meriana ena, ba ipeha kotsing ea litla-morao tse sa hlokahaleng, litšenyehelo tse eketsehileng le tieho ea phekolo e sebetsang.

Ka hona, tlhahlobo e nepahetseng le e bonolo ea KRAS ke motheo oa moralo oa kalafo o ikhethileng.

Bothata ba ho Sibolla: Ho Arola Letšoao la Phetoho

Mekhoa ea setso hangata ha e na kutloelo-bohloko bakeng sa liphetoho tse fokolang tsa lefutso, haholo-holo lisampoleng tse nang le hlahala e tlase kapa ka mor'a ho tlosoa ha khalsiamo. Bothata bo ho khetholla lets'oao le fokolang la DNA le fetotsoeng khahlanong le semelo se phahameng sa mofuta oa hlaha - joalo ka ho fumana nale ka har'a furu. Liphetho tse sa nepahalang li ka lebisa kalafong e fosahetseng le liphellong tse senyehileng.

Tharollo ea Rona: E entsoe ka nepo bakeng sa ho lemoha phetoho e tsitsitseng

KRAS ea rona ea ho Fumana Phetoho ea Liphetoho e kopanya mahlale a tsoetseng pele ho hlola mefokolo ena, e fana ka ho nepahala ho ikhethang le ts'epo bakeng sa tataiso ea kalafo ea mCRC.

Kamoo Theknoloji ea Rona e Tiisang Tshebetso e Phahameng Kateng

• Theknoloji e Ntlafalitsoeng ea ARMS (Sistimi ea Phetoho ea Amplification Refractory): E hahiloe holim'a theknoloji ea ARMS, e kenyelletsa theknoloji ea ntlafatso ea eona ho eketsa ho hlaka ha ho fumanoa.
• Ntlafatso ea Enzymatic: E sebelisa li-endonuclease tse thibelang ho sileha boholo ba nalane ea mofuta oa tlhaho oa genome ea motho, e boloka mefuta ea li-mutant, ka hona e ntlafatsa qeto ea ho lemoha le ho fokotsa keketseho e sa tobang ka lebaka la nalane e phahameng ea genome.
• Ho Thibela Mocheso: Ho hlahisa mehato e itseng ea mocheso ts'ebetsong ea PCR, ho baka ho se lumellane pakeng tsa li-primer tse fetotsoeng le litempele tsa mofuta oa tlhaho, ka hona ho fokotsa bokamorao ba mofuta oa tlhaho le ho ntlafatsa qeto ea ho lemoha.
• Ho ba le kutlo e phahameng: E ​​lemoha ka nepo DNA e fetotsoeng e ka tlase ho 1%.
• Ho nepahala ho Hlollang: E ​​sebelisa maemo a ka hare le enzyme ea UNG ho thibela liphetho tse fosahetseng tse ntle le tse mpe.
• E bonolo ebile e potlakile: E qeta tlhahlobo ka metsotso e ka bang 120, e sebelisa liphaephe tse peli tsa karabelo ho nolofatsa ho lemoha liphetoho tse robeli tse fapaneng, e leng se hlahisang liphetho tse nepahetseng le tse tšepahalang.
• Ho Sebetsana ha Sesebelisoa: E ikamahanya le lisebelisoa tse fapaneng tsa PCR.

Moriana o nepahetseng bakeng sa kankere ea mala o qala ka tlhahlobo e nepahetseng ea limolek'hule. Ka ho sebelisa KRAS Mutation Detection Kit ea rona, laboratori ea hau e ka fana ka liphetho tse hlakileng le tse sebetsang tse bopang tsela ea kalafo ea mokuli ka kotloloho.

Matlafatsa laboratori ea hau ka theknoloji e tšepahalang, e tsoetseng pele—'me u nolofalletse tlhokomelo ea botho e le kannete.

Iteanye le rona: papatso@mmtest.Com

Ithute haholoanyane ka ho kopanya tharollo ena e tsoetseng pele mosebetsing oa hau oa tlhahlobo.

#Mala a mala #Kankere #DNA #Phetoho #Ho nepahala #E reretsweng #Kalafo #Kankere

Ho Notlolla Meriana e Nepahetseng Kankere ea Mala a Maholo

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