Ha tlhahlobo ea nucleic acid e ntse e fetoha tlhokahalo ea kamehla, na u kile ua tobana le liphephetso tsena: li-reagent tse behang kotsing ea ho senyeha nakong ea lipalangoang, mekhoa ea ho bula e sekametseng ho silafatsoa, kapa tahlehelo ea ts'ebetso ka lebaka la lipotoloho tse pheta-phetoang tsa ho hatsela le ho qhibiliha?
Theknoloji ea khale ea "ho hanyetsa lilemo" ea lekholo la lilemo—ho omisa ka vacuum cleaner—hona joale e khanna phetoho e kgolo indastering ya tlhahlobo ya dimolekhule.
Kajeno, ha re hlahlobeng hore naMacroMicroTech (MMT)e sebetsana le lintlha tse 'ne tse kholo tsa bohloko ka eonae sebelisoang hanngoe, e entsoeng ka botlalo e omisitsoe ka ho hatsetsoaskelefo e nyenyanetheknoloji.
I. Ho Khutla ho Hlollang ha Theknoloji ea Khale ea Lilemo tse Lekholo: Nalane e Khutšoanyane ea ho Omisa ka Hoamisoa
Ka 1909, bo-rasaense ba ile ba qala ho sebelisa mokhoa oa ho omisa ka serame ho boloka serum; ka 1919, o ile oa nolofalletsa ho boloka mefuta ea vaerase nako e telele. E kile ea thoholetsoa e le "mohlokomeli oa ts'ebetso ea 'mele," mokhoa oa ho omisa ka serame joale o nchafalitsoe 'me o sebelisoa haholo tlhahlobong ea limolek'hule ea sejoale-joale.
1. Leeto le Boima la Di-Reagent tsa Metsi tsa Setso
Ho tloha ho moetsi ho isa ho mosebelisi oa ho qetela, li-reagent tsa metsi li lokela ho bolokoa tlas'a maemo a thata a ketane e batang nakong eohle ea polokelo le lipalangoang. Tsamaiso e fosahetseng hangata e lebisa ho senyeha ha li-enzyme, ho se tsitse ha lipatlisiso, le likarabelo tse hlōlehileng—bothata bo pheta-phetoang bo tlalehiloeng lithutong tsa koetliso le lithutong tsa linyeoe.
2. Potoloho ea Bophelo ba Li-Reagent tse Metsi tse Kotsing e Kholo
Hang ha e butsoe, lebokose la li-reagent tse hoammeng hangata le mamella mekhahlelo e mengata ea ho qhibiliha, ho sebelisoa le ho hoamisoa hape—ho fihlela e jeoa ka botlalo kapa e lahloa—e leng se fellang ka tahlehelo ea ts'ebetso le tšenyo.
Ho omisa ka serame ho rarolla mathata ana motsong. Ka ho tlosa metsi ka tlas'a vacuum mochesong o tlase, li-enzyme le li-probe li "koaletsoe" boemong bo tsitsitseng - ha e le hantle li hatella khefu holim'a ho senyeha ho fihlela motsotso oa ts'ebeliso.
II. Melemo e mene ea Bohlokoa: Hobaneng ho omisa ka leqhoa e le tharollo e ntle ka ho fetisisa bakeng sa tlhahlobo ea limolek'hule
Li-microsphere tsa MMT tse sebelisoang hanngoe feela, tse entsoeng ka botlalo tse omisitsoeng ka ho hatsetsoa li ntlafalitsoe ho felisa mathata a bohlokoa a indasteri:
III. Katleho ea Theknoloji: Hobaneng MMT e Khethile Micro e Omisitsoeng ka Ho Hatsetsaselika-likoes?
Har'a mefuta e fapaneng e kang ho kenngoa ka har'a vial kapa PCR-tube lyophilization, MMT e bile pula-maliboho oamicro e omisitsoeng ka ho hatsetsaselika-likoesebopehoe finyellang katleho e meraro ea bohlokoa:
uena“E le 'ngoeMicrosphere= Karabelo e le 'Ngoe e Felletseng”
Microsphere e 'ngoe le e 'ngoe ea 2 mm e na le likarolo tsohle
—di-primer, di-enzyme, di-dNTP—ho netefatsa ho nepahala le ho fedisa diphoso tsa ho kopanya di-aliquote.
uenaHo Nchafatsa Molaotheo ka Motsotso o le Mong
Lintho tse khethehileng tse thusang li lumella li-microspheres ho qhibiliha ka potlako ka 50% ho feta mekhoa e tloaelehileng ea ho omisa ka leqhoa.
uenaHo lumellana ha Sebopeho se Butsoeng
E lumellana le mefuta e mengata ea lintho tse sebelisoang le lisebelisoa (mohlala, li-tube tsa PCR tse nang le strip 8, lifomate tsa tšebeliso e le 'ngoe), e netefatsang ho tenyetseha ho pholletsa le lits'ebetso.
IV. Bokamoso ke Hona Joale: Kamoo ho Omisa ka ho Hatsela ho Tla Bopa Bocha Tlhahlobo ea Limolek'hule
Ha tlhahlobo ea limolek'hule e ntse e atamela haufi le libaka tsa tlhokomelo, liteko tsa lapeng, le libaka tsa tlhokomelo ea mantlha ea bophelo bo botle, MMT e se e loketse ka sethala sa eona se matla sa ho omisa ka serame.
Mohlala, litekong tsa phanele ea ho hema, li-microsphere tse omisitsoeng ka ho hatsetsoa li fana ka liphetho tse tšepahalang habonolo, ntle le ketane e batang kapa tlhophiso e rarahaneng.
Protokhole e Tloaelehileng:
Ho AIO800
Ho tloha ho"Matšoenyeho a ketane e batang"ho"Tokoloho ea mocheso oa kamore,"ho tloha ho"Mekhoa e rarahaneng"ho"Ho lemoha mohato o le mong," Theknoloji ea ho omisa ka ho hatsetsa e hlalosa bocha meeli ea botšepehi tlhahlobong ea limolek'hule.
MMT e tla tswela pele ho ntlafatsa tsela ya botsitso, bokgoni, bonolo le boiketlo—e matlafatsa di-diagnostic tse nepahetseng ho itokolla mathateng a nako le sebaka.
Nako ea poso: Tshitwe-26-2025